Download Mouse Models of Cancer: Methods and Protocols by Robert Eferl, Emilio Casanova PDF

By Robert Eferl, Emilio Casanova

This quantity is vital for geneticists, molecular biologists, biochemists, and docs attracted to using mouse versions in melanoma study. contemporary genome reports, including sophisticated genetic engineering strategies, have enormously elevated the worth of utilizing mice for learn on melanoma and different human problems. The chapters of this ebook will aid scientists in deciding on the main appropriate mouse types for his or her learn questions. The publication offers particular methodological info for genetic or chemical induction of other different types of melanoma, histomorphometric melanoma research, and in vivo imaging, in addition to protocols to enquire oncogene habit, immune surveillance, and hallmarks of melanoma reminiscent of angiogenesis or metastasis. 4 review-like articles supply history info on mouse applied sciences and histopathologic variations among mouse and human cancers. The mouse types defined in person chapters will gas the knowledge of melanoma initiation, immune approach roles, tumor angiogenesis, invasion, metastasis, and the relevance of molecular variety saw between human cancers. Written within the hugely winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and warding off identified pitfalls.

Cutting-edge and imaginitive, Mouse versions of Cancers: equipment and Protocols, is a important laboratory source for all researchers, from the graduate point upwards, who examine melanoma and new percentages for its treatment.

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Mouse Models of Cancer: Methods and Protocols

This quantity is key for geneticists, molecular biologists, biochemists, and doctors attracted to using mouse versions in melanoma study. fresh genome reviews, including subtle genetic engineering strategies, have significantly elevated the price of utilizing mice for learn on melanoma and different human problems.

Additional info for Mouse Models of Cancer: Methods and Protocols

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J Thorac Oncol 6(2):244–285. 0b013e318206a221 [doi] 01243894-201102000-00004 [pii] Maeshima A, Miyagi A, Hirai T, Nakajima T (1997) Mucin-producing adenocarcinoma of the lung, with special reference to goblet cell type adenocarcinoma: immunohistochemical observation and Ki-ras gene mutation. Pathol Int 47(7):454–460 Dabbagh K, Takeyama K, Lee HM, Ueki IF, Lausier JA, Nadel JA (1999) IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. J Immunol 162(10):6233–6237 Yamamoto H, Bai YQ, Yuasa Y (2003) Homeodomain protein CDX2 regulates gobletspecific MUC2 gene expression.

The second generation of GEMs for cancer research is based on the targeted modification of endogenous tumor susceptibility genes using homologous recombination [10]. They include knockout mice that are deficient in tumor suppressor genes as well as mice that express oncogenic mutants under the control of the endogenous promoter [11–13]. The first and second generations of GEMs have provided novel insights into the biological significance of the gain-of-function of 50 Kazuhito Sakamoto et al. oncogenes or the loss-of-function of tumor suppressor genes in cancer initiation.

12-211755 Abdullah LH, Wolber C, Kesimer M, Sheehan JK, Davis CW (2012) Studying mucin secretion from human bronchial epithelial cell primary culturesMethods in molecular biology (Clifton. Methods Mol BIol 842:259–277. 1007/978-1-61779-513-8_16 Linnoila RI, Sahu A, Miki M, Ball DW, DeMayo FJ (2000) Morphometric analysis of CC10hASH1 transgenic mouse lung: a model for bronchiolization of alveoli and neuroendocrine carcinoma. Exp Lung Res 26(8):595–615 Giangreco A, Reynolds SD, Stripp BR (2002) Terminal bronchioles harbor a unique airway stem cell population that localizes to the bronchoalveolar duct junction.

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